EndoTrap® HD

Assay Principle

EndoTrap® HD employs a bacteriophage-derived protein ligand with high affinity and specificity for the conserved core region of LPS molecules. As an affinity matrix, the resin binds endotoxin effectively while allowing target analytes to pass through with high recovery. EndoTrap® HD can be applied in batch or column mode, including gravity flow and automated chromatography (higher flow rates than EndoTrap® red).

Intended Use

EndoTrap® HD is intended for in vitro removal of endotoxins (LPS) from research and bioprocessing samples (proteins, peptides, antibodies, nucleic acids, antigens, vaccines, plant extracts, and cell extracts). The resin supports laboratory workflows and scalable purification, including automated liquid chromatography and FPLC.

Key Advantages

• Best-in-class binding capacity > 5,000,000 EU/ml resin
• Reusable more than 10 times
• Requires high salt (0.1 mM free Ca²⁺ or Mg²⁺)
• Compatible with DTT (up to 10 mM)
• Works with DNA, RNA, plant extracts, automated systems
• Leakage ELISA and regulatory documentation available
• Achieves LPS concentration under 0.1 EU/ml

Pack sizes and formats

• EndoTrap® HD 1/1 1× 1 mL prepacked column, equilibration / regeneration / storage buffers (25 mL each)
• EndoTrap® HD 5/1 5× 1 mL prepacked columns, equilibration / regeneration / storage buffers (125 mL each)
• EndoTrap® HD Buffer Kit Equilibration / regeneration / storage buffers (125 mL each)
• EndoTrap® HD Leakage ELISA 96 determinations: coated MTP, POD-antibody, ABTS substrate, standard
• EndoTrap® HD FPLC Column 5 mL (17 × 35 mm) 1× 5 mL FPLC column filled with buffer (replaces LET-HD-FPLC5ML)
• EndoTrap® HD FPLC Column 1 mL (8 × 35 mm) 1× 1 mL FPLC column filled with buffer

Technical Specifications

• Technology: Affinity chromatography with bacteriophage-derived LPS ligand
• Target: Bacterial endotoxin (lipopolysaccharide, LPS)
• Binding capacity: > 5,000,000 EU/ml resin
• Reusability: More than 10 times
• pH range: 4-10
• Buffer compatibility: Requires high salt (0.1 mM free Ca²⁺ or Mg²⁺)
• Sample types: Proteins, peptides, antibodies, DNA/RNA, antigens, plant extracts
• Application modes: Batch, gravity flow, automated chromatography
• Flow rates: Higher than EndoTrap® red; suitable for FPLC

* Measured values for BSA, monoclonal antibody (MAK), and peroxidase (POD).

Citations

1. Harm S, Falkenhagen D, Hartmann J. Endotoxin adsorbents in extracorporeal blood purification: do they fulfill expectations? Int J Artif Organs. 2014;37(3):222-232. PMID: 24744167
2. Arfi ZA, Hellwig S, Drossard J, et al. Polyclonal antibodies for specific detection of tobacco host cell proteins can be efficiently generated following RuBisCO depletion and the removal of endotoxins. Biotechnol J. 2016;11(4):507-518. PMID: 26632519
3. Van Belleghem JD, Merabishvili M, Vergauwen B, et al. A comparative study of different strategies for removal of endotoxins from bacteriophage preparations. J Microbiol Methods. 2017;132:153-159. PMID: 27913133
4. Hietala V, Horsma-Heikkinen J, Carron A, et al. The Removal of Endo- and Enterotoxins From Bacteriophage Preparations. Front Microbiol. 2019;10:1674. PMID: 31396188 Full text (PDF)
5. Silva F, Sitia L, Allevi R, et al. Combined Method to Remove Endotoxins from Protein Nanocages for Drug Delivery Applications: The Case of Human Ferritin. Pharmaceutics. 2021;13(2):229. PMID: 33562060 Full text (PDF)