EndoTrap® - Endotoxin Removal
Our EndoTrap® products efficiently remove endotoxins (lipopolysaccharides/LPS) from a wide range of biological and clinical samples with excellent recovery rates. Sample types include proteins, antibodies, vaccines, nucleic acids, buffers, and cell extracts in aqueous solutions. EndoTrap® has been referenced for elimination of endotoxins in more than 200 publications.a
Advantages of EndoTrap®
- Best-in-class endotoxin binding capacity of >5,000,000 EU/ml
- Removes >99.99% of LPSb
- Achieves LPS concentration of under 0.1 EU/ml
- Excellent sample recovery rate of >95%b
- Non-toxic bacteriophage-derived ligand, no cytotoxic effects
- Quick and easy to use in column (gravity flow) or batch mode
- Re-usable >10 times
- Supports fully automated liquid-chromatographic systems with 5 mL pre-packed FPLC columns
- Larger columns for custom up-scaling are available upon request
EndoTrap® Kits
Our ready-to-use kits are optimized for different binding capacities and buffer conditions, supporting batch and column workflows. See the comparison table below and select from two types of affinity resins offered by Lionex.
EndoTrap® red Kit Family
For selected applications, chelator-compatible, batch and column mode.
View product page →
EndoTrap® HD Kit Family
High-capacity kits for demanding R&D and automated manufacturing.
View product page →
| EndoTrap® red | EndoTrap® HD | |
|---|---|---|
| Binding capacity | 2,000,000 EU per ml of resin | >5,000,000 EU per ml of resin |
| Reusability | ≥3 times | >10 times |
| Sample type | proteins, peptides, antibodies | proteins, peptides, antigens, antibodies, DNA/RNA, plant extracts |
| Sample buffer | compatible with chelators (optional) | requires high salt (0.1 mM free Ca2+ or Mg2+) |
| pH range | 6-9 | 4-10 |
| Workflow | superior for selected applications | superior for automated systems and biomanufacturing processes |
EndoTrap® Equipment
Columns, accessories, and consumables for gravity-flow and automated EndoTrap® chromatography. For a complete list of EndoTrap® equipment and materials, review our product catalogue.
| Product | Description |
|---|---|
| EndoTrap® HD FPLC Column 5 mL | 5 mL resin, pre-filled with buffer |
| EndoTrap® HD FPLC Column 1 mL | 1 mL resin, pre-filled with buffer |
| Empty Columns Large (pack of 5) | For manual packing |
To request columns with custom formats, please contact us here
Endotoxin Services
Expert services offered by Lionex, complementing the EndoTrap® product range.
Removal
| Service | Description |
|---|---|
| Pretesting | Binding assessment with ~1 mg protein |
| Endotoxin removal | Target <100 EU/mg. Includes sample analysis and return. |
Determination
| Service | Description |
|---|---|
| Express | Results within 2 working days (200 μL sample) |
| Standard | Results within 7 working days |
Resources
For detailed advice on buffers, regeneration, and workflows, also see:
EndoTrap® documentation →
EndoTrap® FAQ →
Citations
- Harm S, Falkenhagen D, Hartmann J. Endotoxin adsorbents in extracorporeal blood purification: do they fulfill expectations? Int J Artif Organs. 2014;37(3):222-232. PMID: 24744167
- Arfi ZA, Hellwig S, Drossard J, et al. Polyclonal antibodies for specific detection of tobacco host cell proteins can be efficiently generated following RuBisCO depletion and the removal of endotoxins. Biotechnol J. 2016;11(4):507-518. PMID: 26632519
- Van Belleghem JD, Merabishvili M, Vergauwen B, et al. A comparative study of different strategies for removal of endotoxins from bacteriophage preparations. J Microbiol Methods. 2017;132:153-159. PMID: 27913133
- Hietala V, Horsma-Heikkinen J, Carron A, et al. The Removal of Endo- and Enterotoxins From Bacteriophage Preparations. Front Microbiol. 2019;10:1674. PMID: 31396188
Full text (PDF)
- Silva F, Sitia L, Allevi R, et al. Combined Method to Remove Endotoxins from Protein Nanocages for Drug Delivery Applications: The Case of Human Ferritin. Pharmaceutics. 2021;13(2):229. PMID: 33562060
Full text (PDF)
For an extended list of references, see EndoTrap® HD Regulatory Support File (V04-2021).