EndoTrap® red

Assay Principle

EndoTrap® red employs a bacteriophage-derived protein ligand with high affinity and specificity for the conserved core region of LPS molecules. As an affinity matrix, the resin binds endotoxin effectively while allowing target analytes to pass through with high recovery. EndoTrap® red can be applied in batch or column mode, including gravity flow.

Intended Use

EndoTrap® red is intended for in vitro removal of endotoxins (LPS) from research and bioprocessing samples, including proteins, peptides and antibodies. The resin is designed to support laboratory workflows where low endotoxin levels are critical for assay accuracy and product quality.

Key Advantages

• Optimized for buffers containing calcium chelators
• Binding capacity 2,000,000 EU/ml resin
• Reusable at least 3 times
• Non-toxic bacteriophage-derived ligand
• Quick and easy to use in column (gravity flow) or batch mode
• Achieves LPS concentration under 0.1 EU/ml (repetitive use)

Pack sizes and formats

EndoTrap® red kits contain:

• EndoTrap® red 5x Equilibration Buffer
• EndoTrap® red 5x Regeneration Buffer
• EndoTrap® red Columns

For catalogue numbers and ordering details, see our product catalogue.

Technical Specifications

• Technology: Affinity chromatography with bacteriophage-derived LPS ligand
• Target: Bacterial endotoxin (lipopolysaccharide, LPS)
• Binding capacity: 2,000,000 EU/ml resin
• Reusability: At least 3 times
• pH range: 6-9
• Buffer compatibility: Compatible with chelators (optional)
• Sample types: Proteins, peptides, antibodies
• Application modes: Batch, gravity flow
• Achieves LPS concentration under 0.1 EU/ml (repetitive use)

Citations

1. Van Belleghem JD, Merabishvili M, Vergauwen B, et al. A comparative study of different strategies for removal of endotoxins from bacteriophage preparations. J Microbiol Methods. 2017;132:153-159. PMID: 27913133
2. Hietala V, Horsma-Heikkinen J, Carron A, et al. The Removal of Endo- and Enterotoxins From Bacteriophage Preparations. Front Microbiol. 2019;10:1674. PMID: 31396188 Full text (PDF)
3. Silva F, Sitia L, Allevi R, et al. Combined Method to Remove Endotoxins from Protein Nanocages for Drug Delivery Applications: The Case of Human Ferritin. Pharmaceutics. 2021;13(2):229. PMID: 33562060 Full text (PDF)