Title: Demonstration of Multi-WAP results leading to novel, validated multiplexed, label-free fiberoptic biosensor array system for waterborne pathogen detection.

Link: https://www.igstc.org/home/ongoing_projects

The aim of DEMO-Multi-WAP is to bring to the market a device and ready-to-use test kits (‘plug & play’ cartridges) for waterborne pathogens detection. As evidenced by the success of the Multi-WAP project, there is a clear unmet need to move our technology from TRL 4 (current status) to TRL 6-7 at the end of DEMO-Multi-WAP project. The platform is a multiplexed, rapid, label-free, and real-time method for continuous monitoring multiple waterborne pathogens present in water samples at low cost and high sensitivity (>90%). We will be able to realize this by building upon a highly sensitive array sensor system concept which has been validated for different biomarkers. The array sensor system and the U-bent fiber optic probes (IITM and CBS) are inherently compatible with low-cost interrogation and will be supplemented with further cost- and volume-reducing developments which have already been shown in a lab-context. Apart from relying on existing antibodies at LIONEX, we will further investigate and produce stable biofunctionalized plasma-treated and silanized fibers (IOT and LIONEX) to further boost the performance testing (All partners). A commercial product is the clear end focus of the partners, given that the consortium is made up entirely of SME, startups in incubation, research organizations.

The specific objectives achievable within DEMO-Multi-WAP project are

1. Development and validation of prototype device and ready to use test kits for waterborne pathogens detection.
2. Performance evaluation testing of the platform with minimum 300 samples, achieving a sensitivity of more than 90%.
3. To develop marketable, improved products through successful demonstration of Multi-WAP platform having the robustness and reliability complying with the EU specific directives.

Thus, both disposable sensors cartridges and the optoelectronic device are directly linked to the Multi-WAP project and shall be an excellent demonstration of IGSTC 2+2 program project “Multi-WAP”.

Coordinator: Prof. Dr. Mahavir Singh, LIONEX GmbH