EndoTrap® family

ENDOTOXIN REMOVAL RESINS

EndoTrap® family: Endotoxin removal products are now available from LIONEX, Braunschweig, GERMANY

Notification: The complete EndoTrap® product range as well as EndoGrade® Ovalbumin have been discontinued by Hyglos as of 31 December
2017, instead LIONEX GmbH, Braunschweig, Germany, is now the manufacturer and exclusive supplier of these products.


 

EndoTrap® removes endotoxins (lipopolysaccharides/LPS) from aqueous solutions of a wide range of samples such as proteins, antibodies,
vaccines, nucleic acids, buffers and cell extracts with excellent sample recovery rate.
EndoTrap® has been used for the elimination of endotoxin in more than 200 publications. 

Up-scaling on fully automated liquid-chromatographic systems possible using the 5 ml pre-packed FPLC columns recently introduced by LIONEX. Large columns for up-scaling are also available

New Performance Data for EndoTrap®

A new study (Reich et al Univ Regensburg) describes the efficiency of four endotoxin removal resins from different suppliers including
EndoTrap®. EndoTrap® HD was shown to be the most effective resin for removing endotoxins and recovering protein in comparison to the
other resins tested under the following conditions;

  1. BSA samples [1 ml of 1 mg/ml] in 20mM Borate buffer, 150 mM NaCl, 0.1 mM CaCl2,
    pH 9, containing 15,000 EU/ml were purified with the indicated resins,
  2. BSA samples [1 ml of 1 mg/ml] in 20 mM TRIS, 150 mM NaCl, 0.1 mM CaCl2 pH 7.4, containing 15,000 EU/ml were purified with the
    indicated resins,
  3. BSA samples [1 ml of 1 mg/ml] in 20mM sodium acetate, 150 mM NaCl, 0.1 mM CaCl2, pH 5, containing 15,000 EU/ml were purified with the
    indicated resins,
  4. BSA samples [1 ml of 1 mg/ml] in 20mM MES, 150 mM NaCl, 0.1 mM CaCl2, pH 6, containing 15,000 EU/ml were purified with the indicated resins.

 

Advantages of using Endotrap®

®

®

  • Best-in-class endotoxin binding capacity of > 5,000,000 EU/ml
  • Removes more than 99.99%* of LPS
  • Excellent sample recovery rate >95%*
  • Non-toxic bacteriophage-derived ligand, no cytotoxic effects
  • Re-use more than 10 times
  • Reaching a LPS concentration of under 0.1 EU/ml
  • Quick and easy to use in column (gravity flow) or batch mode
  • Up-scaling on fully automated liquid-chromatographic systems possible using the 5 ml pre-packed FPLC columns recently introduced by LIONEX. Large columns for up-scaling are also available.
EndoTrap® red
> 2,000,000 EU/ml resin
Reusability: 3 times
pH sample buffer : 6-9
Suitable Buffers: chelators
Sample Applications:
Proteins,
Peptides,
Antibodies
EndoTrap® HD
> 5,000,000 EU / ml of resin
Reusability: than 10 times
pH sample buffer: 4-10
Suitable Buffers: Suitable for high salt conditions
(> 150 mM NaCl)
Sample Applications:
Proteins,
Peptides,
Antibodies,
DNA / RNA,
Antigens,
Plant extracts,
Automated systems,
Biomanufacturing processes
*Measured values for BSA, MAK (monoclonal antibody) and POD (peroxidase) with EndoTrap® HD.

 

If you have any questions please contact us

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Centre for Structural Systems Biology (CSSB)

Notkestraße 85, Building 61
22607 Hamburg

+49 00000000
info@lionex.de

Lionex

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Last update:  May 5, 2019 @ 18:26 Lionex GmbH

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