EndoTrap® family
ENDOTOXIN REMOVAL RESINS
EndoTrap® family: Endotoxin removal products are now available from LIONEX, Braunschweig, GERMANY
Notification: The complete EndoTrap® product range as well as EndoGrade® Ovalbumin have been discontinued by Hyglos as of 31 December
2017, instead LIONEX GmbH, Braunschweig, Germany, is now the manufacturer and exclusive supplier of these products.
EndoTrap® removes endotoxins (lipopolysaccharides/LPS) from aqueous solutions of a wide range of samples such as proteins, antibodies,
vaccines, nucleic acids, buffers and cell extracts with excellent sample recovery rate.
EndoTrap® has been used for the elimination of endotoxin in more than 200 publications.
New Performance Data for EndoTrap®
A new study (Reich et al Univ Regensburg) describes the efficiency of four endotoxin removal resins from different suppliers including
EndoTrap®. EndoTrap® HD was shown to be the most effective resin for removing endotoxins and recovering protein in comparison to the
other resins tested under the following conditions;
EndoTrap®. EndoTrap® HD was shown to be the most effective resin for removing endotoxins and recovering protein in comparison to the
other resins tested under the following conditions;
- BSA samples [1 ml of 1 mg/ml] in 20mM Borate buffer, 150 mM NaCl, 0.1 mM CaCl2,
pH 9, containing 15,000 EU/ml were purified with the indicated resins, - BSA samples [1 ml of 1 mg/ml] in 20 mM TRIS, 150 mM NaCl, 0.1 mM CaCl2 pH 7.4, containing 15,000 EU/ml were purified with the
indicated resins, - BSA samples [1 ml of 1 mg/ml] in 20mM sodium acetate, 150 mM NaCl, 0.1 mM CaCl2, pH 5, containing 15,000 EU/ml were purified with the
indicated resins, - BSA samples [1 ml of 1 mg/ml] in 20mM MES, 150 mM NaCl, 0.1 mM CaCl2, pH 6, containing 15,000 EU/ml were purified with the indicated resins.
Advantages of using Endotrap®
®
®
- Best-in-class endotoxin binding capacity of > 5,000,000 EU/ml
- Removes more than 99.99%* of LPS
- Excellent sample recovery rate >95%*
- Non-toxic bacteriophage-derived ligand, no cytotoxic effects
- Re-use more than 10 times
- Reaching a LPS concentration of under 0.1 EU/ml
- Quick and easy to use in column (gravity flow) or batch mode
- Up-scaling on fully automated liquid-chromatographic systems possible using the 5 ml pre-packed FPLC columns recently introduced by LIONEX. Large columns for up-scaling are also available.
EndoTrap® red
> 2,000,000 EU/ml resin
Reusability: 3 times
pH sample buffer : 6-9
Suitable Buffers: chelators
Sample Applications:
Proteins,
Peptides,
Antibodies
> 2,000,000 EU/ml resin
Reusability: 3 times
pH sample buffer : 6-9
Suitable Buffers: chelators
Sample Applications:
Proteins,
Peptides,
Antibodies
EndoTrap® HD
> 5,000,000 EU / ml of resin
Reusability: than 10 times
pH sample buffer: 4-10
Suitable Buffers: Suitable for high salt conditions
(> 150 mM NaCl)
Sample Applications:
Proteins,
Peptides,
Antibodies,
DNA / RNA,
Antigens,
Plant extracts,
Automated systems,
Biomanufacturing processes
> 5,000,000 EU / ml of resin
Reusability: than 10 times
pH sample buffer: 4-10
Suitable Buffers: Suitable for high salt conditions
(> 150 mM NaCl)
Sample Applications:
Proteins,
Peptides,
Antibodies,
DNA / RNA,
Antigens,
Plant extracts,
Automated systems,
Biomanufacturing processes
*Measured values for BSA, MAK (monoclonal antibody) and POD (peroxidase) with EndoTrap® HD.
If you have any questions please contact us
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