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EndoTrap® family

ENDOTOXIN REMOVAL RESINS

EndoTrap® family: Endotoxin removal products are now available from LIONEX, Braunschweig, GERMANY

Notification: The complete EndoTrap® product range as well as EndoGrade® Ovalbumin have been discontinued by Hyglos as of 31 December
2017, instead LIONEX GmbH, Braunschweig, Germany, is now the manufacturer and exclusive supplier of these products.


 

EndoTrap® removes endotoxins (lipopolysaccharides/LPS) from aqueous solutions of a wide range of samples such as proteins, antibodies,
vaccines, nucleic acids, buffers and cell extracts with excellent sample recovery rate.
EndoTrap® has been used for the elimination of endotoxin in more than 200 publications. 

EndoTrap® HD Kit Family

EndoTrap® HD is intended for in vitro quantitative removal of lipopolysaccharide (LPS) from biological samples in aqueous solutions such as proteins, antibodies, cell extracts and nucleic acids. EndoTrap® HD can be used in small scale processes for R&D and large scale processes, like manufacturing. It can be applied in early or late biomanufacturing process steps. EndoTrap® HD is based on hydrophilic, dimensionally stable affinity matrix with excellent pressure/flow characteristics.

 

LET0009 EndoTrap® HD 1/1
LET0010 EndoTrap® HD 5/1
LET0031 EndoTrap® HD Buffer Kit
LET0014 EndoTrap® HD Leakage ELISA
LET-HD-FPLC5ML

 

EndoTrap® HD 1/1

 

LET0009 EndoTrap® HD 1/1

The EndoTrap ® HD 1/1 contains:
1x 1 mL EndoTrap ® HD 1 mL column (prepacked)
1x 25 mL EndoTrap ® HD Equilibration Buffer
1x 25 mL EndoTrap ® HD Regeneration Buffer
1x 25 mL EndoTrap ® HD Storage Buffer
1x Package Insert

 

EndoTrap® HD 5/1

 

LET0010 EndoTrap® HD 5/1

The EndoTrap ® HD 5/1 contains:
5x 1 mL EndoTrap ® HD 1 mL column (prepacked)
1x 125 mL EndoTrap ® HD Equilibration Buffer
1x 125 mL EndoTrap ® HD Regeneration Buffer
1x 125 mL EndoTrap ® HD Storage Buffer
1x Package Insert

 

EndoTrap® HD Buffer Kit

 

LET0031 EndoTrap® Buffer Kit

1x 125 mL EndoTrap ® HD Equilibration Buffer
1x 125 mL EndoTrap ® HD Regeneration Buffer
1x 125 mL EndoTrap ® HD Storage Buffer
1x Package Insert

 

EndoTrap® HD Leakage ELISA

 

LET0014 EndoTrap® HD Leakage ELISA:

96 determinations (12 x 8 well strips & frame)
1. EndoTrap® Leakage ELISA coated MTP: Streptavidin microtiter plate, pre-coated with EndoTrap® ligand-specific monoclonal antibody
2. EndoTrap® Leakage ELISA POD-Antibody: Peroxidase-conjugated monoclonal antibody specific for EndoTrap® ligand (lyophilized)
3. ABTS Substrate, 20 mL (ready to use)
4. EndoTrap® Leakage ELISA Standard: Standard protein (lyophilized)
5. Package insert with working instructions

 

EndoTrap® HD FPLC Column 5 mL

 

LET-HD-FPLC5ML EndoTrap® HD FPLC 5 mL:

1x EndoTrap® HD FPLC column 5mL
1x Package Insert

 

EndoTrap® red Kit Family

EndoTrap® is an affinity matrix intended for removal of lipopolysaccharide (LPS) from biological samples in aqueous solutions such as proteins, antibodies, cell extracts and nucleic acids. EndoTrap® can be employed in both batch and column mode. Especially for the use with buffers containing calcium chelators. This multipurpose product portfolio covers a broad range of conditions with no special equipment or buffer requirements.

LET0001 EndoTrap® red 1/1
LET0002 EndoTrap® red 5/1
LET0032 EndoTrap® red Buffer Kit

EndoTrap® red 1/1

 

LET0001 EndoTrap® red 1/1

The EndoTrap ® red 1/1 contains:
1x 1 mL EndoTrap ® red 1 mL column (prepacked)
1x 25 mL EndoTrap ® red Equilibration Buffer
1x 25 mL EndoTrap ® red Regeneration Buffer
1x Package Insert

 

EndoTrap® red 5/1

 

LET0002 EndoTrap® red 5/1

The EndoTrap ® red 5/1 contains:
5x 1 mL EndoTrap ® red 1 mL column (prepacked)
1x 125 mL EndoTrap ® red Equilibration Buffer
1x 125 mL EndoTrap ® red Regeneration Buffer
1x Package Insert

 

EndoTrap® red Buffer Kit

 

LET0032 EndoTrap® red Buffer Kit

1x 125 mL EndoTrap ® red Equilibration Buffer
1x 125 mL EndoTrap ® red Regeneration Buffer
1x Package Insert

 

New Performance Data for EndoTrap®

A new study (Reich et al Univ Regensburg) describes the efficiency of four endotoxin removal resins from different suppliers including
EndoTrap®. EndoTrap® HD was shown to be the most effective resin for removing endotoxins and recovering protein in comparison to the
other resins tested under the following conditions;

  1. BSA samples [1 ml of 1 mg/ml] in 20mM Borate buffer, 150 mM NaCl, 0.1 mM CaCl2,
    pH 9, containing 15,000 EU/ml were purified with the indicated resins,
  2. BSA samples [1 ml of 1 mg/ml] in 20 mM TRIS, 150 mM NaCl, 0.1 mM CaCl2 pH 7.4, containing 15,000 EU/ml were purified with the
    indicated resins,
  3. BSA samples [1 ml of 1 mg/ml] in 20mM sodium acetate, 150 mM NaCl, 0.1 mM CaCl2, pH 5, containing 15,000 EU/ml were purified with the
    indicated resins,
  4. BSA samples [1 ml of 1 mg/ml] in 20mM MES, 150 mM NaCl, 0.1 mM CaCl2, pH 6, containing 15,000 EU/ml were purified with the indicated resins.

 

Advantages of using Endotrap®

®

®

  • Best-in-class endotoxin binding capacity of > 5,000,000 EU/ml
  • Removes more than 99.99%* of LPS
  • Excellent sample recovery rate >95%*
  • Non-toxic bacteriophage-derived ligand, no cytotoxic effects
  • Re-use more than 10 times
  • Reaching a LPS concentration of under 0.1 EU/ml
  • Quick and easy to use in column (gravity flow) or batch mode
  • Up-scaling on fully automated liquid-chromatographic systems possible using the 5 ml pre-packed FPLC columns recently introduced by LIONEX.Large columns for up-scaling are also available.
EndoTrap® red EndoTrap® HD
> 2,000,000 EU/ml resin > 5,000,000 EU / ml of resin
Reusability: 3 times Reusability: than 10 times
pH sample buffer : 6-9 pH sample buffer: 4-10
Suitable Buffers: chelators Suitable Buffers: Suitable for high salt conditions
(> 150 mM NaCl)
Sample Applications:
Proteins,
Peptides,
Antibodies
Sample Applications:
Proteins,
Peptides,
Antibodies,
DNA / RNA,
Antigens,
Plant extracts,
Automated systems,
Biomanufacturing processes
*Measured values for BSA, MAK (monoclonal antibody) and POD (peroxidase) with EndoTrap® HD.

 

If you have any question please contact us

Lionex GmbH

Lionex Diagnostics and Therapeutics GmbH

Salzdahlumer Straße 196, 38126 Braunschweig, Germany

Lionex

© 2018 Lionex GmbH

Last update:  Mar 9, 2021 @ 17:11 Lionex GmbH

TÜV Rheinland