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Zeitaufgelöste Fluoreszenzdetektion für die integrierte Multiparameter- Analyse von Multiresistenzen beispielgebend bei Tuberkulose

Time-resolved fluorescence detection for integrated multiparameter analysis of multi-resistance in tuberculosis as an example

Multi-resistance has become a significant  health problem in fight against  infectious diseases. The overall goal of this project is to develope a rapid detection system for multi-resistance using the example of tuberculosis infection.


In the technological part, a photonic platform is being researched that should enable tuberculosis diagnostics outside of a laboratory environment through a high degree of integration. The principle of the detection platform is based on time-resolved fluorescence measurement, with which complex optics and wavelength filters can be dispensed off. This is made possible by the use of ps lasers and single-photon avalanche diode arrays (SPAD), whose switching times are optimized for the detection of short-lived fluorochromes. The platform will also be suitable for other multi-parameter analyses of diagnostics, biotechnology and bio-economy. The molecular-biological-biochemical approach consists of a novel detection method that uses a fluorescence quenching effect to literally illuminate the multi-resistance genes by means of a fluorochrome-binding antibody.

Innovation and perspectives

In particular, the method will improve tuberculosis diagnostics and the determination of multi-resistance and contribute to the containment of the disease through accelerated diagnostics on site. However, it is also quickly adaptable for other multi-resistance detections, such as the acute problem of infections in clinical environments which are often accompanied by signs of resistance. The combination of two innovations, the photonic integration of time-resolved fluorescence measurement for short-lived fluorochromes and the biochemical fluorescence quenching antibody assay, are expected to leads to a new detection technology with which complex analyses, as yet inaccesible,  may become greatly simplified and thus widely accessible.

Project Partners:
Lionex GmbH, Braunschweig (Coordinator)

IMMS GmbH, Ilmenau
iC-Haus GmbH, Bodenheim
X-FAB Global Services GmbH, Erfurt
DITABIS Digital Biomedical Imaging Systems AG, Pforzheim
Fraunhofer-Institut für Zelltherapie und Immunologie (IZI), Potsdam
Microfluidic ChipShop GmbH, Jena
Institut für Molekulare Diagnostik und Bioanalytik (IMDB) gGmbH,

Projekt coordination:
Lionex GmbH

Prof. Dr. Mahavir Singh


FLUORESYST  is supported by the Bundesministerium für Bildung und Forschung (BMBF)


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Last update:  Jan 16, 2022 @ 14:10 Lionex GmbH

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